Question 1

How do I reconstitute a peptide vial?

Accepted Answer

Add your chosen volume of bacteriostatic water to the lyophilized peptide vial, swirl gently until the powder dissolves (do not shake), then draw your dose on a U-100 insulin syringe. Use the PeptideCalc reconstitution calculator to find exactly how many units to draw based on your vial strength and water volume.

Question 2

How much bacteriostatic water should I add to a peptide vial?

Accepted Answer

Common recommendations: 10 mg vial → 1 mL (yields 10 mg/mL, 1 unit = 0.1 mg). 40 mg vial → 2 mL (20 mg/mL). 50 mg vial → 2.5 mL (20 mg/mL). Less water creates a more concentrated solution; more water creates a more dilute solution.

Question 3

How do I calculate units from mg for a peptide?

Accepted Answer

Concentration (mg/mL) = vial strength ÷ water volume added. Units to draw = (target mg ÷ concentration) × 100. For example: 5 mg dose from a 10 mg/mL solution = (5 ÷ 10) × 100 = 50 units on a U-100 syringe.

Question 4

What is a U-100 insulin syringe?

Accepted Answer

A U-100 insulin syringe holds 1 mL total volume and is marked in 100 units. Each unit equals 0.01 mL. These are the standard syringes used for subcutaneous peptide injections and are widely available at pharmacies without a prescription.

Question 5

What if my peptide dose exceeds 100 units on a U-100 syringe?

Accepted Answer

A U-100 syringe holds a maximum of 100 units (1 mL). If your dose calculation exceeds 100 units, you should either split the dose across two injections or use less bacteriostatic water to create a more concentrated solution, reducing the number of units needed.